Thermal Destruction of Anti-A1 and Anti-A(A2) from Group O and Group B Serum

摘要

The study has demonstrated that DEAE fractionated IgG anti-A from group O and group B serum could be converted to anti-A(1). The rate of conversion for fractionated IgG was not as rapid as serum, a fact that is consistent with documented thermostability properties of IgG. Scientific literature has suggested that the conversion of anti-A to anti-A(1) may have a practical application in blood banking operations, i.e., differentiation of group A from A subgroups. Evidence from this study leads to the conclusion that the ABO type and thus the immunoglobulin consistency of serum from that type is not as significant as (1) the original anti-A titer and (2) the magnitude of the difference between the anti-A(1) and anti-A(A2) when seeking an antiserum that will not appreciably denature while retaining those properties which enable it to convert to anti-A(1) specificity. (Author)