Spatially Offset Raman Spectroscopy for Surgical Margin Evaluation During Breast Conserving Therapy.

摘要

During year 2 of the project (months 12-24), a SORS probe was designed and tested based on simulation predictions in year 1. It was then employed to acquire spectra from 35 frozen-thawed breast tissue samples in vitro. Spectra from each detector ring were averaged to create a composite spectrum with biochemical information covering the entire range from the tissue surface to approximately 2 mm below the surface, and a probabilistic classification scheme was used to classify these composite spectra as negative or positive margins. This discrimination was performed with 95% sensitivity and 100% specificity, or with 100% positive predictive value and 94% negative predictive value.