Separation of Highly-Active Allergen from the Gaiskii Tularemia Strain No. 15.

摘要

By making use of various methods a number of allergen preparations were obtained from a tularemia microbial mass (vaccine strain). Studies were made of the chemical composition and activity (based on the skin test). The most expressed capacity to cause a cutaneous reaction in sensitized animals was possessed by the preparation obtained by the chloroform method. In a dose of 0.5-1 microgram it caused the same reaction as tularin. Following the fractionation of the chloroform allergen by the method of salting-out with ammonium sulfate three fractions were obtained. Of these I and II were active, and III, consisting mainly of nucleic acids, did not possess the properties of the allergen.