An Examination of the Metabolism of Thiamin by Rat Liver Alcohol Dehydrogenase.

摘要

Thiamin and its thiazole moiety, 5-(2-hydroxyethyl)-4-methylthiazole, have been examined as substrates for purified rat liver alcohol dehydrogenase. Although thiamin itself is a poor substrate for the enzyme, its thiazole moiety is oxidized at a faster rate than ethanol. With incubation times greater than 5 minutes, the major product of the in vitro reaction of the thiazole moiety of thiamin with RLADH is the corresponding acid, 4-methylthiazole-5-acetic acid (thiazole acetic acid).