The Corneal Epithelium: Effects of Anoxia on Krebs Cycle Activity.

摘要

An improved tetrazolium histochemical stain was developed specifically for succinic dehydrogenase (SDH) in corneal epithelium, which is sensitive enough for quantitative photometric measurements. Decreased SDH activity was found associated with moderate oxygen deprivation, i.e., anoxia for 1 and 3 hours, or not less than 5 p. 100 oxygen for 5 hours. Increased SDH activity, or accumulation, was found associated with extreme oxygen deprivation, i.e., anoxia for 5 hours, or less than 5 p. 100 oxygen for 5 hours. Light and electron microscopic-histochemical techniques confirmed that the end product of the SDH reaction is localized at the cell membrane surfaces and in the mitochondria. (Author)