Determination of 11-Hydroxycorticosteroid in Human Urine.

摘要

A fluorometric method for determination of 11-hydroxycorticosteroid in urine as a measure of adrenocortical activity has been adapted for use. Recoveries from pooled urine (N=10) ranged from 94-115%, x = 105%. Day-to-day reproducibility studies (N=10) showed S.D. = plus or minus 14% for the method. Replicate determinations performed on the same day varied by no more than 5%. Twenty-four hour urinary excretion for 20 healthy male volunteers (age 20-60 years) ranged from 84-225 micrograms/day, x = 160 micrograms.