Evaluation of Moloney Murine Sarcoma and Leukemia Virus Complex as a Model for Airborne Oncogenic Virus Biohazards: Survival of Airborne Virus and Exposure of Mice.

摘要

Abstract-Aerosols of the Moloney murine sarcoma virus (MuSV-M) and leukemia virus (MuLV-M) complex (MuSV-M/MuLV-M) were generated from refluxing atomizers and then aged in rotating drums at 21 C holding temperature with relative humidities ranging from 25 to 76%. The MuSV-M and MuLV-M aerosolized from the same tumor extract preparation survived almost equally at the four humidity levels. Both viruses remained viable in the airborne state for at least 2 hours after aerosolization. When mice were exposed to airborne MuSV-M/MuLV-M, no macroscopic lesions were observed in lungs or other tissues examined during the 2-month postexposure period. On the basis of this study, MuSV-M was determined unsuitable as a 'model system' in which a simple aerosol dose response could be used for biohazard evaluation of oncogenic virus aerosols. (Author)