Lack of Aging of GD-Inactivated Subtilisin BPN'.

摘要

To determine whether GD-inactivated subtilisim BPN'ages (loss of pinacolyl group),it was hydrolyzed with alkali and the liberated phosphonic acid derivative was partitioned between the two phases of an isobutanol-benzene:trichloroacetic acid-HC1mixture. Its partition ratio, O/A,where O is the amount in the organic phase and A is the amount in the aqueous phase,was 27.0. Pinacolyl methylphosphonic acid,prepared from GD,had a ratio of 16.4,whereas methylphosphonic acid,also prepared from GD,had a ratio of 0.1. The partition ratio of 27.0agrees well with that of 26.0obtained from GD-inactivated bovine erythrocyte acetylcholinesterase under conditions in which it is known not to age. It is concluded that GD-inactivated subtilisin BPN'does not age.