Studies on Triton X-100 Detergent Micelles.

摘要

Triton X-100 micelle formation at 25 C was studied by use of sedimentation equilibrium and fluorescence spectroscopic techniques. The apparent molecular weight of the major Triton X-100 micelle was found to be 81 250, indicating a micelle number of 125. A micelle number of 121 was obtained with fluorescence titration experiments, which showed one molecule of 1-anilino-8-naphthalene sulfonate binding per micelle with an apparent association constant of 90,000 M. The fluorescent tritration experiments also indicated the presence of another TX-100 binding species of variable size. (Author)