An Automated DMFB Method for the Determination of Urinary Amino Nitrogen.

摘要

A method using 2,4-dinitrofluorobenzene (DNFB) is described for the automatic determination of urinary amino nitrogen. Based on existing manual methods, it introduces reagents that are easily adaptable to automated techniques. A mean urinary amino nitrogen value of 77.1 mg amino N/gm creatinine was obtained for a group of healthy male subjects, compared to a range of 52 to 80 mg reported by other investigators. Direct comparison of analytical results obtained by the automated method and a manual DNFB method on several urine samples yielded a mean difference of 1.34% between the methods. (Author)