Cryobiologic Approaches to the Recovery of Immunologic Responsiveness of Murine and Human Mononuclear Cells.

摘要

Recent advances in immunology have allowed for the use of various measurements of lymphocyte function to determine recovery of subsets of lymphocytes following freezing and thawing. Murine T cells are known to respond to phytohemagglutinin (PHA), whereas B cells respond to bacterial endotoxin (LPS). In the human, in addition to mitogen responses, T cells may be measured by their ability to form spontaneous rosettes with sheep erythrocytes (E-rosettes) B cells and monocytes form sheep erythrocyte-antibody-complement rosettes (EAC rosettes). These systems have been used in the present study to determine the effects of various cryobiologic procedures on the recovery of immune responsiveness.