Composition and Function of Phospholipids in Plasmodium Knowlesi.

摘要

The lipid extracts of normal and Plasmodium knowlesi-infected rhesus erythrocytes and of the parasite itself have been examined for phospholipid composition on an animal-to-animal basis. Phosphatidylinositol (PI), phosphatidylcholine (PC), and phosphatidylethanolamine (PE), represented larger percentages in the parasite than in the host. Sphingomyelin (SM) and phosphatidylserine (PS) averaged less than 20% and 33% respectively in the parasite than in the infected erythrocyte. The phospholipid distribution and the ratio of cholesterol-to-cholesterol ester were determined for plasmas isolated from normal, fasted, and Plasmodium knowlesi-infected monkeys. Compared to normal plasmas, the infected plasmas showed patterns with slight increases in PC and large decreases in lysophosphatidylcholine (LPC). A successful high-pressure liquid chromatography separation of LPC, PE, PI, PS, SM and phosphatidic acid has been achieved with synthetic samples.