Purification of Acetylcholinesterase from House Fly Brain by Affinity Chromatography.

摘要

Acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) from the heads of house flies (Musca domestica L.) was purified by affinity chromatography. The enzyme was adsorbed from the crude extracts on an affinity column containing trimethyl(p-aminophenyl) ammonium chloride hydrochloride (Ki approxmately equal to 1.7 .ten to the minus 4th power M), covalently linked to Sepharose 4B, then eluted with a solution of a selective reversible inhibitor, 1-5-bis (4-allyl dimethyl ammoniumphenyl)-pentan-3-one dibromide.