The Preparation and Characterization of a Thymic Independent Antigen: epsilon-Dinitrophenyl-L-Lysine Ficoll.

摘要

The preparation of a hapten-polysaccharide conjugate, epsilon-dinitrophenyl-L-lysine-Ficoll, is described. Conditions yielding different substitution ratios were studied. Ultracentrifuge and gel filtration studies have shown a very broad range size heterogeneity. The conjugates were shown to elicit both immediate and delayed skin reactions in guinea pigs previously immunized with dinito-phenylated keyhole limpet hemocyanin. The presence of antibody forming cells in the spleens of mice immunized with the conjugates was established by the demonstration of plaque-forming cells when assayed with trinitrophenylated sheep red blood cells. The immunogenicity, as shown by the plaque-forming cell response, was shown to be markedly dependent on the size of the conjugates.