Quantitative Isolation of Messenger RNA from Free and Bound Polysomes of Control and Infected Rat Liver.

摘要

A procedure is described for the quantitative separation of poly A-rich mRNA from free and bound ribosomes. Detergent, rather than phenol-chloroform, were utilized for RNA extraction and the mRNA from free and bound ribosomes was isolated on oligo (dT)-cellulose columns. Evidence of the effectiveness of this procedure was demonstrated through electron microscopy, sucrose density gradient polyribosomal patterns, and RNA distribution studies. These techniques were utilized to study the effects of Streptococcus pneumoniae infection on hepatic transcription translation rates. Increases in transcription rates directed toward the bound ribosomes were observed.