Effect of Vaccination Schedule on Immune Response of 'Macaca mulatta' to Cell Culture-Grown Rocky Mountain Spotted Fever Vaccine.

摘要

The effect of vaccination schedule on the immune response of Macaca mulatta to formalin-inactivated chicken embryo cell culture (CEC)-grown Rickettsia rickettsii vaccine was studied. Humoral antibody measured by microagglutinatiion and indirect immunofluo-rescence and resistance to challenge with 1000 plaque-forming units of yolk sac-grown R. rickettsii were assessed. Seroconversion was noted in all monkeys after the first dose of vaccine. Only monkeys given two doses of vaccine at a 30-day interval showed an increase in antibody titer during the period before challenge. The two-dose schedules appeared to induce the highest degree of resistance to challenge, as indicated by unaltered hematological parameters and body temperature in monkeys.