Impaired Gluconeogenesis in Isolated Hepatocytes from S. pneumoniae Infected Rats: Effect of Infection on Gluconeogenesis.

摘要

The gluconeogenic capacity of single cell suspension of hepatocytes isolated from control and S. pneumoniae infected rats was determined. It is shown that (1) gluconeogenesis from pyruvate and lactate is reduced by 35-42% in hepatocytes isolated from agonal rats; (2) addition of ethanol fails to return glucose synthesis to control levels; and (3) the activity of a rate limiting gluconeogenic enzyme, phosphoenolpyruvate carboxykinase is reduced by 47% in livers of agonal rats. It is proposed that the decrease in PEPCK activity, rather than a lack of reducing equivalents may account for the agonal breakdown in glucose synthesis during severe infections. (Author)