Recovery of Alveolar Macrophages from Rhesus and Cynomolgus Monkeys by Lung Lavage.

摘要

A lung lavage technique has been developed to recover alveolar macrophages from rhesus and cynomolgus monkeys. Sterile saline was injected through an endotracheal tube in anesthetized monkeys and then lung wash fluids containing leukocytes were withdrawn. The lung wash fluids from each monkey routinely contained more than 20 x 10 to the 6th power leukocytes of which 80% were alveolar macrophages. Lung lavage has been performed weekly for 6 wk in both species of monkey with no ill effects. This technique has many applications in the study of infection and pulmonary defense mechanisms. (Author)