Model for Disseminated Intravascular Coagulation: Bacterial Sepsis in Rhesus Monkeys.

摘要

Disseminated intravascular coagulation (DIC) is a hemorrhagic syndrome frequently encountered as a complication in severe Gram-negative bacterial sepsis. An animal model for sepsis-associated DIC was developed in order to permit study of the appearance and development of this syndrome in relation to the entire disease process. Rhesus monkeys (4-6 kg) were infected by intravenous injection of 10 to the 9th power S. typhimurium. Fibrinolytic system activation was indicated by the appearance of fibrin degradation products. Kinin system activation was evidenced by decreases in both prekallikrein and kininogen. Changes in laboratory tests suggestive of subclinical DIC were also noted in infected monkeys which did not develop a rash. Pathological evidence of DIC was obtained through observation of numerous fibrin thrombi in the kidneys of the only monkey which died in the course of infection. Occurrence of DIC in association with this experimental infection in rhesus monkeys was established on the basis of clinical, laboratory, and pathologic criteria. Expression of the syndrome on day 1-2 following infection correlated with the period of increasing bacteremia.