Production of Malaria Vaccine by Artificial Feeding of Mosquitoes.

摘要

The report is divided into three chapters. (1) Infection of mosquitoes by feeding under artificial conditions. Experimental requirements for artificially feeding mosquitoes are described along with the necessary special apparatus which was designed and built in our laboratory. (2) Determination of the best mouse strain for carrying gametocytes and production of high numbers of sporozoites. It is demonstrated that the NMRI mouse is clearly the best choice for proliferation of both these stages of the Plasmodium berghei parasites. (3) Determination of Exoerythrocytic stages of P. berghei. Preliminary experiments involving tissue culture of embryonic rat tissue where sporozoites develop into mature schizonts are described. (Author)