Laser Raman Spectroscopy of Ultraviolet-Induced Cataracts in Rabbits and Monkeys

摘要

The Raman spectra of normal rabbit and monkey lenses have been recorded over the range of 0-1/4000 cm. Spectral results indicate that protein conformation is predominantly beta-pleated sheet. Polarization measurements and assignments for most of the bands have been made; the spectral region for water indicates extensive hydrogen bonding. A nitrogen laser, operating at 337 nm, was used to produce cataracts in rabbit and monkey lenses; the Raman spectra of the cataractous lenses were also recorded. Spectral changes, observable in the water region, are correlated with a mechanism of ultraviolet cataractogenesis.