Identification of Druggable Proteins Regulating Receptor Recycling in Breast Cancer Cells.

摘要

Several recycling regulatory proteins are overexpressed in breast cancer patients and promote the progression of breast cancer. Increased receptor recycling leads to elevated receptors on the plasma membrane, thereby augments signaling cascades important in cancer progression and drives tumor aggressiveness. Inhibition of the altered recycling pathway in breast cancer cells would thus dampen oncogenic signaling and increase the efficacy of therapy. To screen druggable proteins regulating receptor recycling, we proposed to monitor EGFR trafficking using a newly developed AP-tag system. We found that while the AP-tag labeling worked for receptor endocytosis studies as described in the original literatures, it could not efficiently label the recycled EGFR. Therefore, this technique is not suitable for studying EGFR recycling as originally proposed.