Isolation and Characterization of Erythrocyte and Parasite Membranes from Rhesus Red Cells Infected with P. knowlesi.

摘要

Two immunogenic antigens common to host cell membranes of rhesus erythrocytes infected by two strains of P. knowlesi, were shown to be reactive with antibodies from patients acutely exposed to P. falciparum, P. vivax, as well as rhesus or squirrel monkeys made immune to infection with P. cynomolgi and P. falciparum respectively. In rhesus monkeys one of the immune components (component 13) clearly correlates with protective immunity. The immune components are also shared by P. falciparum merozoites and host cell membranes from P. cynomolgi-infected erythrocytes. The data indicate that at least one of the antigens, component 13, is an interspecies antigen. The Plasmodium specific antigens can be labeled metabolically by both amino acids and sugar precursors, showing them to be glycoproteins. Their molecular weights and isoelectric points fall in the ranges 90,000-55,000D and pH4.5-5.2, respectively. The Plasmodium specific antigens described are exposed at the external surfaces of infected erythrocytes.