Studies on the Mechanism of Action of the 'in vitro' PGB Sub x Effect. 4. The Effect of Order of Addition of PGB Sub x to Assay System

摘要

The mechanism of action of PGB sub x in RLM oxidative phosphorylation was studied by varying the sequence of addition of PGB sub x. When PGB sub x was added to RLM preexposed to hypotonic media at 27 deg no restoration of phosphorylation was observed. When RLM were exposed to hypotonic conditions for 30 seconds before the addition of PGB sub x and subsequently exposed to hypotonic conditions, the resulting phosphorylation was 60% of that observed in the normal system. From these results it is concluded that PGB sub x functions in the in vitro PGB sub x RLM assay system as a protective agent rather than a restorative agent.