A Computer Program to Process Spectrophotometric Analytical Data Associated with Curvilinear Absorbance/Concentration Relationships

摘要

A computer program designed to provide a simple and accurate method for evaluating spectrophotometric assay data accommodates both linear and nonlinear standard curves. The program requires manual entry by keyboard of absorbance and concentration values for the standard curve and absorbance values for the test samples. It will process up to 4 dilutions of each test sample and thus it can accommodate batches of samples containing widely differing concentrations of test compound. A regression equation (up to 4th degree) is calculated to describe the standard curve with concentration as the independent variable and absorbance as the dependent variable. Concentrations in unknown samples are calculated from absorbance by using a method of successive approximations. A hard copy data printout lists input data, concentration of test substance in each sample, statistical data concerning the estimate of the standard curve, and a plot of the standard curve.