Heat-Induced Changes in Perfused Rat Liver

摘要

In order to determine the time periods at which heat-induced structural changes occurred, isolated rat livers were perfused for different time intervals at 41 - 43 C with oxygenated Krebs-Ringer bicarbonate containing .14% glucose. Structural changes occurred after 60 minutes at 41 C, 30-45 minutes at 42 C and 15 minutes at 43 C. Under these perfusion conditions, structural changes were detected before bile production or enzyme leakage reflected changes. The data suggest a continuum of events depending on the time and temperature of exposure, leading to the same end point: loss of endothelium, vacuolization, loss of microvilli, and formation of flocculent densities in mitochondria. (Author)