Development of Monoclonal Antibodies to Trypanosoma brucei rhodesiense Antigens.

摘要

This report, covering work performed from 1 September 1979-28 February 1982,describes methdology of tissue culture, hybridization procedures, immunization schedules and antibody assays developed to produce monoclonal antibodies to variant specific surface antigens of Trypanosoma b. rhodesiense organisms of the Walter Reed Army Trypanozoon antigenic type (WRATat) serodeme. Monoclonal antibodies have been produced to WRATats 2-19. These reagents will be critical in assessment of antigenic variation and in potential vaccine development programs. (Author)