Serological Screening Test for Any Botulinum Toxin Type

摘要

Methods are available to test samples directly for A, B or E botulinum toxin, If the toxin type is known. If the type is unknown, testing is complicated in that separate tests for the three types become necessary. This need for multiple tests on one specimen is a distinct disadvantage when many samples are to be examined. The present work was concerned with adapting one of the published ELISA methods into a screening procedure which would give a positive reaction regardless of the toxin type in the sample. Method II was tired by substituting mixtures of antitoxins for the single antitoxin of the type specific ELISA. The advantage of the method over I was the need for only one enzyme-labeled reagent since (anti-AT2)* reacts with rabbit IgG of different antitoxic specificities.