Chemical Carcinogen (Hydrazine et al.) Induced Carcinogenesis of Human Diploid Cells in Vitro.

摘要

Polynuclear hydrocarbons or hydrazine analogues do not have an absolute requirement for binding to DNA in order to elicit a carcinogenic event. Furthermore, PNH like BP enter the nucleus via a lipoprotein complex where the BP is oxygenated and then interacts with the genetic material,(DNA). The adduct formed is 7 beta-BPEDI-dG. Benzamide does not alter the binding of the perceived ultimate carcinogen to dG but eliminates the carcinogenic response. The period of heightened response to the carcinogen appears to be 3 hours into S phase of the cell type. The modifying effect of TPA or Benzamide appears to alter the nuclear non-histone proteins protecting the cellular DNA. (Author)