Next Generation of Advanced Laser Fluorescence Technology for Characterization of Natural Aquatic Environments.

摘要

The project research addresses our long-term goal to develop an analytical suite of the Advanced Laser Fluorescence (ALF) methods and instruments to improve our capacity for characterization of aquatic environments. The ALF technique (Chekalyuk and Hafez, 2008) uniquely combines spectrally and temporally resolved measurements of the laser-stimulated emission (LSE) to provide assessments of key variables, including chlorophyll a (Chl a), chromophoric dissolved organic matter (CDOM), and phycobiliprotein- containing phytoplankton and cyanobacteria. The pump-during-probe measurements of variable fluorescence, Fv/Fm, yield assessments of phytoplankton photophysiological status. An extensive series of ALF measurements in diverse water types has demonstrated ALF utility as an integrated tool for aquatic research and observations. The ALF integration into the major oceanographic programs is currently in progress, including the California Current Ecosystem Long Term Ecological Research (CCE LTER, NSF) and California Cooperative Oceanic Fisheries Investigations (CalCOFI, NOAA).