Study of the Immunologic Response to Second Heterotypic Bluetongue Virus Infection in Mice.

摘要

The first phase of the study involved the development of 2 immunological techniques, (1) an avidin/biotin-enhanced enzyme-linked immunosorbent assay (A/B-ELISA) and (2) an avidin/biotin-enhanced immunoperoxidase staining procedure, for the detection and measurement of serological responses of mice to bluetongue virus (BTV) infection and the demonstration of BTV antigen in mouse tissues. Results indicated that the A/B-ELISA is an effective assay for serum antibody produced against BTV in the mouse; and that BTV antigen is detectable in infected mouse tissues with the avidin/biotin-enhanced immunoperoxidase technique. These observations along with immunoabsorbent studies suggest that much of the response to heterotypic infections is directed against the secondarily infecting virus serotype. These results are in contrast with previous views, based largely on in vitro serum neutralization tests, that infection of an animal by 1 serotype of a virus should not alter the host immune response to infection by a different serotype of that virus.