Conditions for the Chemical and Physical Inactivation of the K. Fu. Strain of the Agent of Creutzfeldt-Jakob Disease

摘要

The unusual resistance of the unconventional viruses to inactivation by the commonly used disinfectants has led to a high degree of apprehension regarding patients with any form of dementia. The rapid adaptation of a newly acquired isolate of the agent of Creutzfeldt-Jakob Disease (CJD) to mice made possible this large scale study of its heat and chemical stability. The agent showed a decrease in titer of approximately two logs following incubation at 80C for 30 minutes with no additional loss at 80C for up to 500 minutes. There was greater than a three log decrease in titer at 100C for thirty minutes and temperatures of 115 and 130 C completely inactivated the agent. Treatment with sodium hypochlorite at three concentrations (0.33% 0.66% and 1.31%) showed inactivation of greater that 99 percent at each. Crude agent preparations were not inactivated by sodium dedecysulfate at detergent to protein ratios up to 4:1. These results suggest that hospital supplies which resist autoclaving may be adequately disinfected by autoclaving.