Detection and Quantitation of T-2 Mycotoxin Using a Simplified Protein Synthesis Inhibition Assay

摘要

A simple, rapid and sensitive bioassay is described for the detection and quantitation of T-2 mycotoxin using a protein synthesis assay in cultured cells. Increased sensitivity of the cells of the mycotoxin occurs with time up to approximately 60 min. Time and dose response curves demonstrate that an average of 10 to 20 ng/ml T-2 is sufficient to cause 50% inhibition of protein synthesis in tissue culture cells. A wide range of tissue culture cells having varied type, tissue, and species sources, and growth characteristics were tested using this system. All showed approximately the same sensitivity to the mycotoxin. Using a slight modification of the procedure, suspended cultures of mitogen-stimulated lymphocytes also demonstrated an equal degree of sensitivity to the mycotoxin. By simple changing the labeled precursor, the inhibition of RNA, DNA and protein synthesis by T-2 mycotoxin can be compared. Although T-2 has little effect of RNA synthesis, DNA and protein synthesis are equally inhibited.