Karnovsky-Osmium as a Primary Fixative for Electron Microscopy

摘要

A combined Karnovsky-osmium tetroxide primary fixative is proposed for transmission electron microscopy. This fixative, hypothetically, seems to overcome the drawbacks of sequential fixation techniques. Evaluation of this combined fixative reveals very acceptable fixation of hepatocytes, lymphocytes, and fibroblasts, as well as various organelles within these cells. (Author)