Special Techniques for the Separation of Hemopoietic Cells: Elutriation

摘要

Several basic methods of cell separation have been developed for the isolation of a purified or enriched cell population from peripheral blood or bone marrow. Counterflow centrifugation-elutriation (CCE; centrifugal elutriation or counter-streaming centrifugation is the cell separation method discussed in this chapter). In this technique, cells are entered into a chamber with a fluid-flow field in opposition to a centrifugal force. Based on cell size and density, the cells either exit the chamber with the fluid flow or remain in suspension within the chamber under the influence of the centrifugal force field. By either increasing the flow rate or decreasing the centrifugal force field, various cell populations can be selectively eluted from the chamber. This chapter discusses the use of CCE in cell separation, along with recent advancements and limitations of the technique.