Viability of Stored Rabbit Erythrocytes Carrying Number C3c

摘要

The purpose of these studies was to 1) explore the usefulness of a rabbit model to quantitate RBC preservation injury in vivo and to 2) evaluate the importance of RBC-bound C3 in the preservation injury. Several technical aspects relating to homologous transfusions of rabbit RBC were investigated during this study period. These included the method of Cr-51 labeling of the preserved RBC and the comparison of single or double labeling techniques for assessing RBC biability. Preliminary data obtained with double labeling method indicated that 99mTc might be a useful label for fresh autologous RBC. The biological data revealed that ADSOL additive improved the survival of RBC in the initial storage period, but that the viability was not maintained during prolonged storage. Destruction of the complement from plasma of the donor units by heat-treatment (to avoid C3 accumulation to RBC membrane) appeared to have adverse effects on RBC survival. It was thought that heat-treatment produced immune complexes. Additional studies are required to evaluate the role of bound C3 in the preservation injury.