Induction of Immunity to Toxins with Anti-Idiotypic Antibody

摘要

Anti-idiotypic antibodies (antibodies directed at the combining site of an antibody molecule) are being tested in a new method for safe and efficient vaccination to substances which are too toxic for direct inoculation. Exotoxin A, an extracellular toxin produced by Pseudomonas aeruginosa is being used as the model toxic substance. A panel of monoclonal mouse antibodies to exotoxin A were prepared in NMRI and balb/c mice, consisting of one IgG1, one IgA and six IgM hybridoma cell lines. Control monoclonals were developed from an existing panel of anti-rickettsia monoclonals of NMRI allotype. The monoclonals were purified by preparative centrifugation and column chromatography and were used to prepare anti-idiotypic antisera in mice and rabbits. High-titered anti-idiotypic antisera to one IgM anti-exotoxin monoclonal, TC-31, was raised in C57B1/6 mice. The antiserum was specific for TC-31 and noncross-reactive with the other monoclonals. High-titered anti-idiotypic antisera to each monoclonal have been prepared in rabbits.