Benzamide Derivatives as Protective Agents against the Action of Xenotoxic Agents on Human Cells

摘要

In addition to finding that benzamide can intervene in carcinogen induced neoplastic transformation that is cell cycle dependent, we also developed a procedure for detecting the presence of a malignant phenotype in sarcoma tumor tissue. Monoclonal antibodies (McAb) were developed against the transformed phenotype that cross reacted with the chemically transformed cells. We were able also to use this McAb for the detection of sarcomatous invasive lesions in human tissue in the presence of normal stroma. Using these procedures described above combined with the analysis of competitive inhibitory kinetics of poly(ADP-ribose) polymerase activity we have now been able to identify eleven of these inhibitors that interfere in the neoplastic transformation process, (to be published in 1984-85). During this time period we were also able to establish human skin xenografts on nude mice and apply these technologies to human skin in vivo situations. We have also introduced a new technology for examining modification of DNA by the carcinogen, i.e. post labeling of the modified DNA that only requires 2 x 10 to the 6th power cells and ca. 190-200 ng of DNA.