Mainframe Computer Programs for Rapid Analysis of Hybridoma Screening Data

摘要

Most laboratories that currently produce large numbers of hybridomas grow them and screen their media by immunoassay in 96-well plates. Robotic systems are commercially available for sterile sampling, feeding and expansion of hybridomas. The authors have written a program package in microsoft Pascal that converts the records for a Dynatech Autoprep automated hybridoma sampler and a Flow, Multiskan enzyme immunoassay reader to an ASCII database format, merges them, and creates tables which can be sorted by any parameter (eg. source plate/well, target plate/well, or assay result) and a histogram from which the best responding hybridomas can be selected.