Safety and Immunogenicity Testing of a Pilot Polysaccharide Vaccine Preparation to Pseudomonas aeruginosa

摘要

We prepared and tested a high-molecule weight polysaccharide antigen from three different immunotype strains of Pseudomonas aeruginosa. These materials were tested in humans for safety and immunogenicity. All three vaccines were found to be safe in doses between 100-250 mcg. They were also found to be immunogenic, inducing antibody responses in 40-90% of immunized persons. These antibodies were also found to be functional in their ability to kill the bacterim in conjunction with white blood cells. In addition, we showed that these vaccines were able to induce and effect on T-cells of immunized animals and humans. This T-cell response may be an important component of the overall immune response to these antigens that results in protective immunity.