Molecular Toxicology of Chromatin: The Role of Poly(ADP-Ribose) in Gene Control

摘要

This report pertains to the following research projects: Chemical and macromolecular structure of poly(ADP-ribose)1. HPLC-isolation of poly(ADP-ribose.). 2. Fractionation, size analysis branching of poly (ADP-ribose) by HPLC and chemical analysis of subunits. 3. Mathematical model of polymerization of ADP-ribose. II. Biosystems. III. Molecular studies on purified poly(ADP-ribosylation in cellular and subcellular systems. III. Molecular studies on purified poly(ADP-ribose) polymerase system. 1. DNA-association of benzamide. 2. The role of lysine residues in the catalysis and DNA binding of poly(ADP-ribose)polymerase. IV. Cell transformation and poly ADP-ribosylation. 1. Inhibition of carcinogen initiated transformation. 2. Ultraviolet light induced transformation and its inhibition.