Mutagenic Screening of Three Dyes for Marker Grenades in the Salmonella Reversion Assay, the L5178Y/TK +/- Mouse Lymphoma Assay, and in vivo Sister Chromatid Exchange in Mice

摘要

Two dyes, C.I. Solvent Yellow No. 33, and a C.I. Solvent Green No. 3 - C.I. Solvent Yellow No. 33 mixture were tested for mutagenicity in the Salmonella Reversion Assay, the L5178Y/TK (VK) Mouse Lymphoma Assay, and for Sister Chromatid Exchange (SCE) in vivo in mice. The in vitro mutagenicity assays were performed both with and without exogenous activation provided by Aroclor induced rat liver S-9. A >99.9% pure sample of the yellow dye (2-(2-quinolyl)-1,3-indandione) was also tested with and without exogenous activation in the Salmonella Reversion Assay and the L5178Y/TK +/- Mouse Lymphoma Assay. Neither C.I. Solvent Yellow No. 33 nor the C.I. Solvent Green No. 3 - C.I. Solvent Yellow No. 33 mixture were positive in inducing in vivo SCE. All three dyes were tested in the standard plate incorporation test in seven strains TA100, TA102, TA104, TA1535, TA1537, TA1538, and TA98. The dyes were negative with and without exogenous activation in TA98, TA1535, and TA1538.