Characterization of Human Neuroblastoma Cell Lines Established before and after Therapy

摘要

Six new cell lines have been established from human neuroblastomas. Cell line SMS-KAN, from primary tumor before therapy, and line SMS-KANR, from bone marrow after chemotherapy and radiotherapy, were established from the same patient. Cell lines SMS-KCN (from primary tumor before any therapy) and SMS-KCNR (from bone marrow after chemotherapy) were established from another patient. Two other lines (SMS-MSN and SMS-SAN) were established from different patients before any therapy was given. Cell lines established from recurrent disease after chemotherapy (SMS-KANR and SMS-KCNR) had significantly shorter doubling times and increased plating efficiencies compared to those of cell lines derived from the same patient before chemotherapy (SMS-KAN and SMS-KCN). All cell lines contained tyrosine hydroxylase, aromatic l-amino acid decarboxylase, and dopamine-beta-hyrdoroxlase. Measureable amounts of choline acetylfransferase were also detected in SMS-KAN and SMS-MSN to be pseudodiploid with modal numbers of 46 and deletions of the short are of chromonsome 1; SMS-MSN had a modal number of 57-58 chromosomes. All cell lines had double-minute chromosomes, except SMS-KANR, which had abnormally banding regions. These new cell lines provide in vitro models of neuorblastoma suitable for the study of differences in neroblastoma cell populations that proliferate after therapy. Keywords: Karyotyping.