Effect of Soman on Protein Phosphorylation in Brain

摘要

Studies were conducted to determine the effect of soman on protein phosphorylation in rat brain. In previous studies, we found that soman treatment caused a transient decrease in protein kinase C but no change in calcium/phospholipid-dependent protein kinase. In present studies we found that treatment with soman had no effect on the in vitro activity of either the cyclic AMP-dependent protein kinase or the cyclic GMP-dependent protein kinase. In previous studies, we found that cholinergic dependent mechanisms increased the phosphorylation of an acidic 80kDa protein in slices of the rat neo-cortex. In the present studies, cholinergic dependent changes in protein phosphorylation were found in the cerebellum, but not in the striatum. Studies were also conducted which enabled the development of methods to study the effect of soman on protein phosphorylation in vivo. Additionally, studies were conducted which indicate that treatment with soman can cause a dramatic increase in cyclic AMP in the rat neo-cortex. Keywords: Toxicity; Response biology. RA 5; Soman; Protein; Phosphorylation; Brain. (kt)