Properties of Stabilized Sub-Micron Phospholipid Vesicles

摘要

The principal focus of research activity has been the characterization of phospholipid vesicles formed with polymerizable disulfide phosphatidylcholines. Structures with the polymerizable groups in both the alpha and terminal positions of the side chain fatty acids have been synthesized and the resulting polymerized structures characterized. The authors have discovered that it is possible to produce very small and highly stable polymerized micellular structures using short chain phosphatidylcholines. Extremely stable phospholipid vesicles can be produced using long chain fatty acids polymerized in the alpha position. The authors have also studied novel synthetic glycolipids in incorporated into phospholipid vesicle structures. These synthetic glycolipids modify the lipid phase behavior substantially and produce vesicles which are stable to lyophylization. The details of these studies have been prepared for publication, and are given in the attached preprint entitled Modification of Lipid Phase Behavior With Membrane Bound Cryoprotectants by Raymond P. Goodrich, Tracy M. Handel, and John D. Baldeschwieler.