Sex Differences in the Recovery of Brain Acetylcholinesterase Activity Following a Single Exposure to DFP (Diisopropylphosphofluoridate)

摘要

Male and female C57BL, DBA, and C3H mice were injected intraperitioneally with a single 6.33 mg/kg dose of diisopropyl-phosphofluoridate (DFP). The time course of recovery of acetylcholinesterase (AchE) activity as well as effects on choline acetyltransferase (ChAT) activity and brain muscarinic and nicotinic receptors were measured. DFP treatment did not affect ChAT activity or the muscarinic an nicotinic receptors were measured. DFP treatment did not affect ChAT activity or the muscarinic and nicotinic receptors. Near control levels of AChE activity were regained in female mice within the first 20 days. However, levels of whole brain AChE acitivity remained depressed for as long as 40 days following a single dose of DFP in male mice. An analysis of the recovery of AChE acitivity in several brain regions indicated that control activity was regained in striatrum, hindbrain, and hippocampus, but not in cortex, midbrain, and hypothalamus. These data are discussed in terms of potential neurotoxicity induced by a single dose of DFP. Keywords: Acetylcholinesterase; Diisopropylphosphofluoridate; Muscarinic receptors; Choline acetyltransferase; Nicotinic receptors; Sex differences; Organic phosphorus compounds.