Cloning of Acetylcholinesterase Gene in a Microbial Vector

摘要

Techniques to distinguish between the activities of human acetylcholinesterase and pseudocholinesterase have been developed. Human neuroblastoma cell lines have been shown to synthesize human acetylcholinesterase and the level of enzyme within these cells can be induced by treatment of cells with sodium butyrate or dibutyryl cAMP. These cell lines should be suitable as a source of mRNA for the cloning of the human gene into E. coli.