Development of In vitro Isolated Perfused Porcine Skin Flaps for Study of Percutaneous Absorption of Xenobiotics

摘要

This report describes the development of a novel in vitro alternative animal model for use in dermatology and cutaneous toxicology. A single pedicle, axial pattern, island-tubed skin flap was created in crossbred Yorkshire weanling pigs in one surgical procedure and then transferred 2 or 6 days later to a computer-controlled temperature-regulated perfusion chamber for 10 to 12 hr studies. The development of this two-stage surgical procedure is fully described. Because the skin of weanling Yorkshire swine has similar anatomy, biochemistry, and permeability to topical agents, the impact of these findings on human dermal risk assessment studies in cutaneous toxicology and on the development of transdermal drug delivery systems may be substantial. This preparation would be a humane alternative animal model for studies in cutaneous toxicology, physiology, oncology, and percutaneous drug absorption and metabolism. Keywords: Biological absorption, Histology. (kt)