Light and Electron Microscopic Demonstration of B-Lymphocytes with a New Mg++/ATPase Method. (Reannouncement with New Availability Information).

摘要

Helper T-lymphocytes are readily demonstrated for light microscopy by an enzyme cytochemical method for nonspecific esterase with the substrate alpha-naphthyl butyrate acetate. It was recently shown that a modification of this esterase method also demonstrates immature and cultured helper T cells. Although Harigaya et al. were able to demonstrate B-cells in B lymphomas and in some smears of whole blood, they were unable to demonstrate B lymphocytes in enriched leukocyte preparations. Studies in our laboratories have shown that a new method for the demonstration of myofibrillar ATPase could be also used to show B cells as long as the fixative contained ATP and the cobalt sulfide end product was intensified and stabilized by very brief treatment with silver methenamine solution in a microwave oven. In the present study it was found that the addition of magnesium to our incubation medium greatly enhanced the demonstration of the b-cell plasma membrane enzyme and that the incubation time could be reduced from 1 hour to 5 minutes by microwave irradiation of the medium.