Ca2+ Ionophoretic Activity of Oligomers of Prostaglandin B1 with Isolated Hepatocytes

摘要

Chemically synthesized oligomers (dimers, trimers and tetramers) of 15-dehydropostaglandin B1 and 16, 16' dimethyl-15-dehydroprostaglandin B1 (16,16'diMePGB1) are effective Ca2+ ionophores with isolated mitochondria and in artificial systems. The trimer of 16,16'diMePGB1 mediated a dose dependent Ca2+ efflux from impact rat hepatocytes; at 9.2 uM oligomer, Ca2+ was released primarily from the mitochondrial pool but at higher concentrations from other cellular pools. The 16,16'deMePGB1 trimer did not alter Ca2+ release mediated by epinephrine suggesting that the PGB1 oligmoer interacts at a different site. The oligomer also caused an activation.